Production of High-purity Minicircle-DNA for Use in Gene Transfer


The present invention relates to a highly efficient method for the production and purification of minicircle-DNA.

Category

Life Sciences

Projectnumber

P0711963

Inventor

Dr. Peter Mayrhofer

Patent Status

  • EP1620559B1
  • US8647863B2
  • AU2004236352B2
  • EP2213744A1
  • US20140065705A1
  • GB1307075.0
  • Additional patent application claiming even better coordination of production and purification porcesses filed in 2013

Description

The present invention relates to a highly efficient method for the production and purification of minicircle-DNA. Based on the coordinated and consecu-tively conducted in vivo processes of recombination, restriction and subsequent affinity purification, the technology provides highly pure minicircle-DNA with an optimised safety profile for direct application in gene therapy and vaccination. The technology was developed by Vienna based Researchers

Application

  • Non-viral gene therapy and vaccination
  • minicircle or plasmid purification

Benefits

  • Combination of minicircle production and purification work flow
  • Consecutive complementary processing steps
  • Optimised safety profile of minicircles
  • High product purity (99 % and higher)

Cooperation options

  • License Agreement
  • collaboration

Contact

Dipl.-Ing. Dr. Manfred Lampl
Schutzrechtsmanagement
+ 43 1 501 75 - 553